What is The product all about?
This product is a buffering agent (acidic buffer) commonly used by molecular biologists to adjust the pH of a solution or stabilize the pH. Commercially available, this product is Tris with HCl added. It can be in used in common buffer recipes such as:
CTAB DNA extraction buffer
Leammli buffer for SDS-PAGE
Tris HCl Buffer Solution
Determining whether to use tris HCl or tris base for these commonly used recipes depends on the protocol you’re working with. When you need to adjust the pH of your solution, you can use the conjugate acidic or basic component (Harvey, n.d.). For example, if using a protocol calling for tris HCl, you can adjust the pH using tris base.
Other protocols involving the product include
DNase Inactivation in RNase A Solution Protocol
The loading buffer for the IPTG Induction and Extraction of Proteins
Radio Immunoprecipitation Assay (RIPA) Cell Lysate Preparation
The TE Buffer component in performing Electrotransformation of Agrobacterium tumefaciens
I have seen some protocols call for Tris Cl and others call for Tris HCl, what is the difference?
After browsing different buffer protocols, you might be wondering what is the difference between tris Cl and tris HCl? There is no difference between tris Cl and tris HCl. They are the same. The recipes both call for the same thing: Dissolving tris into water and adjusting the pH with HCl.
Be cautious about adjusting the pH when working with tris. The pH of tris will change if your working temperature changes. To prevent pH fluctuation, adjust your pH in the same temperature you’re carrying out your work.